2920. Synthetic Biology Gone Out of Control

By Emily Baumgartner, The New York Times, May 14, 2018

Keoni Gandall, in his laboratory at home in Palo Alto, Calif. He barely earned a high school diploma, he said, and was kicked out of a local science fair for reckless genetic engineering. Photo: Erin Brethauer for The New York Times. 

Earlier this year, at Body Hacking Con in Austin, Tex., a biotech executive injected himself with what he hoped would be a herpes treatment. (Verdict: No.) His company already had live-streamed a man injecting himself with a home-brewed treatment for H.I.V. (His viral load increased.)

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Keoni Gandall, in his laboratory at home in Palo Alto, Calif. He barely earned a high school diploma, he said, and was kicked out of a local science fair for reckless genetic engineering.

In a recent interview, Mr. Gandall, now 18 and a research fellow at Stanford, said he only wants to ensure open access to gene-editing technology, believing future biotech discoveries may come from the least expected minds.

But he is quick to acknowledge that the do-it-yourself genetics revolution one day may go catastrophically wrong.

“Even I would tell you, the level of DNA synthesis regulation, it simply isn’t good enough,” Mr. Gandall said. “These regulations aren’t going to work when everything is decentralized — when everybody has a DNA synthesizer on their smartphone.”

The most pressing worry is that someone somewhere will use the spreading technology to create a bioweapon.

Already a research team at the University of Alberta has recreated from scratch an extinct relative of smallpox, horsepox, by stitching together fragments of mail-order DNA in just six months for about $100,000 — without a glance from law enforcement officials.

The team purchased overlapping DNA fragments from a commercial company. Once the researchers glued the full genome together and introduced it into cells infected by another type of poxvirus, the cells began to produce infectious particles.

To some experts, the experiment nullified a decades-long debate over whether to destroy the world’s two remaining smallpox remnants — at the Centers for Disease Control and Prevention in Atlanta and at a research center in Russia — since it proved that scientists who want to experiment with the virus can now create it themselves.

The study’s publication in the journal PLOS One included an in-depth description of the methods used and — most alarming to Gregory D. Koblentz, the director of the biodefense graduate program at George Mason University — a series of new tips and tricks for bypassing roadblocks.

“Sure, we’ve known this could be possible,” Dr. Koblentz said. “We also knew North Korea could someday build a thermonuclear weapon, but we’re still horrified when they actually do it.”

Experts urged the journal to cancel publication of the article, one calling it “unwise, unjustified, and dangerous.” Even before publication, a report from a World Health Organization meeting noted that the endeavor “did not require exceptional biochemical knowledge or skills, significant funds or significant time.”

But the study’s lead researcher, David Evans, a virologist at the University of Alberta, said he had alerted several Canadian government authorities to his poxvirus venture, and none had raised an objection.

Many experts agree that it would be very difficult for amateur biologists of any stripe to design a killer virus on their own. But as more hackers trade computer code for the genetic kind, and as their skills become increasingly sophisticated, health security experts fear that the potential for abuse may be growing.

“To unleash something deadly, that could really happen any day now — today,” said Dr. George Church, a researcher at Harvard and a leading synthetic biologist. “The pragmatic people would just engineer drug-resistant anthrax or highly transmissible influenza. Some recipes are online.”

“If they’re willing to inject themselves with hormones to make their muscles bigger, you can imagine they’d be willing to test more powerful things,” he added. “Anyone who does synthetic biology should be under surveillance, and anyone who does it without a license should be suspect.”

Authorities in the United States have been hesitant to undertake actions that could squelch innovation or impinge on intellectual property. The laws that cover biotechnology have not been significantly updated in decades, forcing regulators to rely on outdated frameworks to govern new technologies.

The cobbled-together regulatory system, with multiple agencies overseeing various types of research, has left gaps that will only widen as the technologies advance.

Academic researchers undergo strict scrutiny when they seek federal funding for “dual-use research of concern”: experiments that, in theory, could be used for good or ill. But more than half of the nation’s scientific research and development is funded by nongovernmental sources.

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In 2013, a quest to create a glowing plant via genetic engineering drew almost half a million dollars through Kickstarter, the crowdfunding website.

“There really isn’t a national governance per se for those who are not federally or government funded,” said Dr. William So, a biological countermeasures specialist at the Federal Bureau of Investigation.

Instead, he said, the agency relies on biohackers themselves to sound the alarm regarding suspicious behavior.

“I do believe the F.B.I. is doing their best with what they have,” said Dr. Thomas V. Inglesby, director of the Johns Hopkins Center for Health Security in Baltimore.

“But if you really want to do this, there isn’t a whole lot stopping you.”

Underground Experimenters

The F.B.I. has befriended many white-hat biohacking labs, among them Genspace in Sunset Park, Brooklyn. Behind an inconspicuous steel door on a gritty, graffiti-lined street, biohackers-in-training — musicians, engineers, retirees — routinely gather for crash courses in genetic engineering.

Participants in “Biohacker Boot Camp” learn basic technical skills to use in homegrown genetics projects, like concocting algae that glows.

“The double helix is the most iconic image of the 20th century, perhaps rivaled only by the mushroom cloud,” the bootcamp’s leader, Michael Flanagan, said to a recent class.

Genspace’s entryway resembles a college dorm room, complete with sagging couch, microwave, mini-fridge. But the lab itself is palatial: two stories of white brick walls, industrial kitchen counters marked with dry-erase notes, shelves towering with glassware and reagents.

It’s a significant upgrade for Genspace. Daniel Grushkin, the co-founder, used to host bacterial experiments in his living room over pizza and beer.

The group later moved into a rental for creatives — roboticists, organic fashion designers, miniature-cupcake makers — and constructed a makeshift lab using old patio screen doors. It was Mr. Grushkin who reached out to the F.B.I.

“People might be calling you because we are nonscientists doing science in a busted-up old building,” he recalled telling bureau agents. “But we aren’t a meth lab, and we aren’t bioterrorists.”

Mr. Grushkin has become a trailblazer in biohacking risk management, in part because he recognizes that letting neophytes manipulate live organisms is “less like a ‘hackerspace,’ more like a pet store.”

He has posted community guidelines, forbidden infectious agents in the lab, and accepted a grant of almost $500,000 to design security practices for some four dozen similar labs across the country.

Most of them report not having heard so much as a greeting from the F.B.I. At many, the consequence for breaking safety guidelines is simply the loss of membership — leaving the perpetrator to experiment in isolation, but still among thousands of enthusiasts huddled online in Facebook groups, email listservs and Reddit pages.

Many find their inspiration in Josiah Zayner, a NASA scientist turned celebrity biohacker who straps a GoPro camera to his forehead and streams experiments on himself from his garage. He’s the man who tried to make his muscles bigger.

“This is just normal Scotch packing tape,” Mr. Zayner, chief executive of a biohacking start-up called The Odin, told his YouTube audience one summer night, muttering expletives as he stripped the top layer of skin from his forearm. “This is Day 1 of my experiment to genetically engineer myself.”

In an interview, Mr. Zayner conceded that among his biohacking followers, an accident — not a premeditated offense — was conceivable.

“I guess I can see why they don’t let the entire public have access to Ebola,” he said. “The risk is, if they’re working with Ebola and their house burns down, the Ebola could somehow get out.”

Even Mr. Zayner is apprehensive of the movement he helped begin; he plans to include live frogs in The Odin’s D.I.Y.-Crispr kits to encourage his followers to experiment on animals instead of themselves — or others.

“I have no doubt that someone is going to get hurt,” he said. “People are trying to one-up each other, and it’s moving faster than any one of us could have ever imagined — it’s almost uncontrollable. It’s scary.”

A Biological Arms Race

If nefarious biohackers were to create a biological weapon from scratch — a killer that would bounce from host to host to host, capable of reaching millions of people, unrestrained by time or distance — they would probably begin with some online shopping.

A site called Science Exchange, for example, serves as a Craigslist for DNA, a commercial ecosystem connecting almost anyone with online access and a valid credit card to companies that sell cloned DNA fragments.

Mr. Gandall, the Stanford fellow, often buys such fragments — benign ones. But the workarounds for someone with ill intent, he said, might not be hard to figure out.

Biohackers will soon be able to forgo these companies altogether with an all-in-one desktop genome printer: a device much like an inkjet printer that employs the letters AGTC — genetic base pairs — instead of the color model CMYK.

A similar device already exists for institutional labs, called BioXp 3200, which sells for about $65,000. But at-home biohackers can start with DNA Playground from Amino Labs, an Easy Bake genetic oven that costs less than an iPad, or The Odin’s Crispr gene-editing kit for $159.

Tools like these may be threatening in the wrong hands, but they also helped Mr. Gandall start a promising career.

At age 11, he picked up a virology textbook at a church book fair. Before he was old enough for a driver’s permit, he was urging his mother to shuttle him to a research job at the University of California, Irvine.

He began dressing exclusively in red polo shirts to avoid the distraction of choosing outfits. He doodled through high school — correcting biology teachers — and was kicked out of a local science fair for what was deemed reckless home-brew genetic engineering.

Mr. Gandall barely earned a high-school diploma, he said, and was rebuffed by almost every college he applied to — but later gained a bioengineering position at Stanford University.

“Pretty ironic, after they rejected me as a student,” he said.

He moved to East Palo Alto — with 14 red polo shirts — into a house with three nonbiologists, who don’t much notice that DNA is cloned in the corner of his bedroom.

His mission at Stanford is to build a body of genetic material for public use. To his fellow biohackers, it’s a noble endeavor.

To biosecurity experts, it’s tossing ammunition into trigger-happy hands.

“There are really only two things that could wipe 30 million people off of the planet: a nuclear weapon, or a biological one,” said Lawrence O. Gostin, an adviser on pandemic influenza preparedness to the World Health Organization.

“Somehow, the U.S. government fears and prepares for the former, but not remotely for the latter. It baffles me.”